Charles G. Hoogstraten
Assistant Professor
- B.S. 1990, Michigan State University, Chemistry & Biochemistry
- Howard Hughes Medical Institute Predoctoral Fellowship, 1990-1995
- Ph.D. 1995, University of Wisconsin - Madison
- Helen Hay Whitney Postdoctoral Fellow, 1995-1998, University of Colorado, Boulder
- Visiting Postdoctoral Fellow/Junior Research Specialist, 1998-2002, UC Davis, Chemistry
hoogstr3@msu.edu
302D Biochemistry Building
Michigan State University
East Lansing, MI 48824-1319
Office: 517-353-3978
FAX: 517-353-9334
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Charles G. Hoogstraten Research Interests continued
A second major focus is the protein-RNA recognition events that are important in the regulation of alternative pre-mRNA splicing in eukaryotes. Alternative splicing is emerging as a major contributor to the regulation of gene expression, and also plays a dominant role in generating diversity in the proteome, which can exceed the diversity of the genome by orders of magnitude. Sequences in the pre-mRNA exons and introns can either favor or disfavor the usage of nearby splice sites when recognized by modular RNA-binding proteins of the SR family. SR proteins are modular molecules whose RNA-binding affinity and specificity emerge in a nonlinear way from the characteristics of individual RNA-binding domains. We will use a combination of NMR studies of domain orientation and ligand-induced folding, EPR in combination with site-directed spin labeling for studies of global interdomain structure, and solution thermodynamics using isothermal titration calorimetry (ITC) to unravel the physicochemical bases of this key RNA-protein recognition event.
Full text of research interests