Laurie S. Kaguni
University Distinguished Professor
- B.A. 1974, University of California, San Diego
- Ph.D. 1980, University of California, Los Angeles
- American Cancer Society, Inc. Postdoctoral Research Fellow, 1980-1983, Stanford University
- American Cancer Society, Inc. Junior Faculty Research Awardee, 1986-1988
- Director, Biochemistry Research Trainee Program, 1998-present
- Distinguished Faculty Award, 2002, 2003; MSU College of Natural Science
- University Distinguished Professor, 2007
- Director, MSU Center for Mitochondrial Science and Medicine, 2008
319 Biochemistry Building
Michigan State University
East Lansing, MI 48824-1319
Office: 517-353-6703
Lab: 517-353-4918
FAX: 517-353-9334
Publication search:
Laurie S. Kaguni Recent Publications
Adán C, Matsushima Y, Hernández-Sierra R, Marco-Ferreres R, Fernández-Moreno MA, González-Vioque E, Calleja M, Aragón JJ, Kaguni LS, Garesse R. Mitochondrial transcription factor B2 is essential for metabolic function in Drosophila melanogaster development. J Biol Chem. 2008 Feb 28; [Epub ahead of print]
Martínez-Azorín F, Calleja M, Hernández-Sierra R, Farr CL, Kaguni LS, Garesse R. Overexpression of the catalytic core of mitochondrial DNA polymerase in the nervous system of Drosophila melanogaster reduces median life span by inducing mtDNA depletion. J Neurochem. 2007 Nov 12; [Epub ahead of print]
Fernández-Moreno MA, Farr CL, Kaguni LS, 2007. Garesse R. Drosophila melanogaster as a model system to study mitochondrial biology. Methods Mol Biol. 372:33-49.
Matsushima Y, Adán C, Garesse R, Kaguni LS. 2007. Functional analysis by inducible RNA interference in Drosophila melanogaster. Methods Mol Biol. 372:207-17.
Ziebarth TD, Farr CL, Kaguni LS. 2007. Modular architecture of the hexameric human mitochondrial DNA helicase. J Mol Biol. Apr 13; 367(5): 1382-91. Epub 2007 Feb 7.
Matsushima Y, Kaguni LS. 2007. Differential phenotypes of active site and human autosomal dominant progressive external ophthalmoplegia mutations in Drosophila mitochondrial DNA helicase expressed in Schneider cells. J Biol Chem. Mar 30 ;282(13): 9436-44. Epub 2007 Jan 31.
Sanchez-Martinez A, Luo N, Clemente P, Adan C, Hernandez-Sierra R, Ochoa P, Fernandez-Moreno MA, Kaguni LS, Garesse R. 2006. Modeling human mitochondrial diseases in flies. Biochim Biophys Acta. Sep-Oct; 1757(9-10): 1190-8. Epub 2006 May 13. Review.
Fan L, Kim S, Farr CL, Schaefer KT, Randolph KM, Tainer JA, Kaguni LS. 2006. A novel processive mechanism for DNA synthesis revealed by structure, modeling and mutagenesis of the accessory subunit of human mitochondrial DNA polymerase. J Mol Biol. May 19; 358(5): 1229-43. Epub 2006 Mar 15.
Garesse R, Kaguni LS. 2005. A Drosophila model of mitochondrial DNA replication: proteins, genes and regulation. IUBMB Life. Aug; 57(8): 555-61. Review.
Luoma PT, Luo N, Loscher WN, Farr CL, Horvath R, Wanschitz J, Kiechl S, Kaguni LS, Suomalainen A. 2005. Functional defects due to spacer-region mutations of human mitochondrial DNA polymerase in a family with an ataxia-myopathy syndrome. Hum Mol Genet. Jul 15; 14(14): 1907-20. Epub 2005 May 25.
Matsushima Y, Adan C, Garesse R, Kaguni LS. 2005. Drosophila mitochondrial transcription factor B1 modulates mitochondrial translation but not transcription or DNA copy number in Schneider cells. J Biol Chem. Apr 29; 280(17): 16815-20. Epub 2005 Mar 3.
Luo N, Kaguni LS. 2005. Mutations in the spacer region of Drosophila mitochondrial DNA polymerase affect DNA binding, processivity, and the balance between Pol and Exo function. J Biol Chem. Jan 28; 280(4): 2491-7. Epub 2004 Nov 10.
Kaguni LS. 2004. DNA polymerase gamma, the mitochondrial replicase. Annu Rev Biochem. 73: 293-320. Review.
Matsushima Y, Garesse R, Kaguni LS. 2004. Drosophila mitochondrial transcription factor B2 regulates mitochondrial DNA copy number and transcription in schneider cells. J Biol Chem. Jun 25; 279(26): 26900-5. Epub 2004 Apr 1.
Farr CL, Matsushima Y, Lagina AT 3rd, Luo N, Kaguni LS. 2004. Physiological and biochemical defects in functional interactions of mitochondrial DNA polymerase and DNA-binding mutants of single-stranded DNA-binding protein. J Biol Chem. Apr 23; 279(17): 17047-53. Epub 2004 Jan 30.
Farr CL, Kaguni LS. 2002. Purification strategies for Drosophila mtDNA replication proteins in native and recombinant form. Mitochondrial single-stranded DNA-binding protein Methods Mol Biol. 197: 285-94. No abstract available.
Farr CL, Kaguni LS. 2002. Purification strategies for Drosophila mtDNA replication proteins in native and recombinant form. DNA polymerase gamma. Methods Mol Biol. 197: 273-83. No abstract available.
Iyengar B, Luo N, Farr CL, Kaguni LS, Campos AR. 2002. The accessory subunit of DNA polymerase gamma is essential for mitochondrial DNA maintenance and development in Drosophila melanogaster. Proc Natl Acad Sci U S A. Apr 2;99(7):4483-8. Epub 2002 Mar 26.
Ugalde C, Ochoa P, Perez ML, Fernandez-Moreno MA, Calleja M, Alahari A, Kaguni LS, Garesse R. 2001. Identification of a proximal promoter region critical for the expression of the beta-F1-ATPase gene during Drosophila melanogaster development. Mitochondrion. Oct;1(3):225-36.
Maier D, Farr CL, Poeck B, Alahari A, Vogel M, Fischer S, Kaguni LS, Schneuwly S. 2001. Mitochondrial single-stranded DNA-binding protein is required for mitochondrial DNA replication and development in Drosophila melanogaster. Mol Biol Cell. Apr;12(4):821-30.
Fan L, Kaguni LS. 2001. Multiple regions of subunit interaction in Drosophila mitochondrial DNA polymerase: three functional domains in the accessory subunit. Biochemistry. Apr 17;40(15):4780-91.
Lefai E, Calleja M, Ruiz de Mena I, Lagina AT 3rd, Kaguni LS, Garesse R. 2000. Overexpression of the catalytic subunit of DNA polymerase gamma results in depletion of mitochondrial DNA in Drosophila melanogaster Mol Gen Genet. Sep;264(1-2):37-46.
Lefai E, Fernandez-Moreno MA, Alahari A, Kaguni LS, Garesse R. 2000. Differential regulation of the catalytic and accessory subunit genes of Drosophila mitochondrial DNA polymerase. J Biol Chem. Oct 20;275(42):33123-33.
Lefai E, Fernandez-Moreno MA, Kaguni LS, Garesse R. 2000. The highly compact structure of the mitochondrial DNA polymerase genomic region of Drosophila melanogaster: functional and evolutionary implications. Insect Mol Biol. Jun;9(3):315-22.
Ruiz De Mena I, Lefai E, Garesse R, Kaguni LS. 2000. Regulation of mitochondrial single-stranded DNA-binding protein gene expression links nuclear and mitochondrial DNA replication in drosophila. J Biol Chem. May 5;275(18):13628-36.
Ruiz de Mena I, Fernandez-Moreno MA, Bornstein B, Kaguni LS, Garesse R. 1999. Structure and regulated expression of the delta-aminolevulinate synthase gene from Drosophila melanogaster. J Biol Chem. Dec 24;274(52):37321-8.
Wang Y, Kaguni LS. 1999. Baculovirus expression reconstitutes Drosophila mitochondrial DNA polymerase. J Biol Chem. Oct 8;274(41):28972-7.
Fan L, Sanschagrin PC, Kaguni LS, Kuhn LA. 1999. The accessory subunit of mtDNA polymerase shares structural homology with aminoacyl-tRNA synthetases: implications for a dual role as a primer recognition factor and processivity clamp. Proc Natl Acad Sci U S A. Aug 17;96(17):9527-32.
Farr CL, Wang Y, Kaguni LS. 1999. Functional interactions of mitochondrial DNA polymerase and single-stranded DNA-binding protein. Template-primer DNA binding and initiation and elongation of DNA strand synthesis. J Biol Chem. May 21;274(21):14779-85.
Wang Y, Farr CL, Kaguni LS. 1997. Accessory subunit of mitochondrial DNA polymerase from Drosophila embryos. Cloning, molecular analysis, and association in the native enzyme. J Biol Chem. May 23;272(21):13640-6.
Lewis DL, Farr CL, Wang Y, Lagina AT 3rd, Kaguni LS. 1996. Catalytic subunit of mitochondrial DNA polymerase from Drosophila embryos. Cloning, bacterial overexpression, and biochemical characterization. J Biol Chem. Sep 20;271(38):23389-94.
Olson MW, Wang Y, Elder RH, Kaguni LS. 1995. Subunit structure of mitochondrial DNA polymerase from Drosophila embryos. Physical and immunological studies. J Biol Chem. Dec 1;270(48):28932-7.
Lewis DL, Farr CL, Kaguni LS. 1995. Drosophila melanogaster mitochondrial DNA: completion of the nucleotide sequence and evolutionary comparisons. Insect Mol Biol. Nov;4(4):263-78.
Thommes P, Farr CL, Marton RF, Kaguni LS, Cotterill S. 1995. Mitochondrial single-stranded DNA-binding protein from Drosophila embryos. Physical and biochemical characterization. J Biol Chem. Sep 8;270(36):21137-43.
Williams AJ, Kaguni LS. 1995. Stimulation of Drosophila mitochondrial DNA polymerase by single-stranded DNA-binding protein. J Biol Chem. Jan 13;270(2):860-5.
Lewis DL, Farr CL, Farquhar AL, Kaguni LS. 1994. Sequence, organization, and evolution of the A+T region of Drosophila melanogaster mitochondrial DNA. Mol Biol Evol. May;11(3):523-38.
Williams AJ, Wernette CM, Kaguni LS. 1993. Processivity of mitochondrial DNA polymerase from Drosophila embryos. Effects of reaction conditions and enzyme purity. J Biol Chem. Nov 25;268(33):24855-62.
Olson MW, Kaguni LS. 1992. 3'-->5' exonuclease in Drosophila mitochondrial DNA polymerase. Substrate specificity and functional coordination of nucleotide polymerization and mispair hydrolysis. J Biol Chem. Nov 15;267(32):23136-42.
Kaguni JM, Kaguni LS. 1992. Enzyme-labeled probes for nucleic acid hybridization. Methods Biochem Anal. 36:115-27. Review. No abstract available.
Kaguni LS, Olson MW. 1989. Mismatch-specific 3'----5' exonuclease associated with the mitochondrial DNA polymerase from Drosophila embryos. Proc Natl Acad Sci U S A. Sep;86(17):6469-73.
Lehman IR, Kaguni LS. 1989. DNA polymerase alpha. J Biol Chem. Mar 15;264(8):4265-8. Review. No abstract available.
Wernette CM, Conway MC, Kaguni LS. 1988. Mitochondrial DNA polymerase from Drosophila melanogaster embryos: kinetics, processivity, and fidelity of DNA polymerization. Biochemistry. Aug 9;27(16):6046-54.
Kaguni LS, Lehman IR. 1988. Eukaryotic DNA polymerase-primase: structure, mechanism and function. Biochim Biophys Acta. Jul 13;950(2):87-101. Review. No abstract available.
Wernette CM, Kaguni LS. 1986. A mitochondrial DNA polymerase from embryos of Drosophila melanogaster. Purification, subunit structure, and partial characterization. J Biol Chem. Nov 5;261(31):14764-70.
Kaguni LS, DiFrancesco RA, Lehman IR. 1984. The DNA polymerase-primase from drosophila melanogaster embryos. Rate and fidelity of polymerization on single-stranded DNA templates. J Biol Chem. Jul 25;259(14):9314-9.
Kaguni LS, Rossignol JM, Conaway RC, Banks GR, Lehman IR. 1983. Association of DNA primase with the beta/gamma subunits of DNA polymerase alpha from Drosophila melanogaster embryos. J Biol Chem. Aug 10;258(15):9037-9.
Kaguni LS, Rossignol JM, Conaway RC, Lehman IR. 1983. Isolation of an intact DNA polymerase-primase from embryos of Drosophila melanogaster. Proc Natl Acad Sci U S A. Apr;80(8):2221-5.
Kaguni LS, Clayton DA. 1982. Template-directed pausing in in vitro DNA synthesis by DNA polymerase a from Drosophila melanogaster embryos. Proc Natl Acad Sci U S A. Feb;79(4):983-7.
Kaguni LS, Kaguni JM, Ray DS. 1981. Replication of M13 oriC bacteriophages in Escherichia coli rep mutant is dependent on the cloned Escherichia coli replication origin. J Bacteriol. Feb;145(2):974-9.