Gavin E. Reid
Research Interests (also see Reid Lab Pages)
The mixture complexity, dynamic range and diversity of transcriptional, translation and post translational modifications associated with protein expression, as well as the complex array of specific protein-protein interactions involved in the regulation of cellular function, presents a significant analytical challenge to mass spectrometry (MS) based proteomic methods employed for characterization of the hitherto unknown functional role of the thousands of genes identified from recent genome sequencing initiatives.
Research in our laboratory is broadly directed toward the development and application of novel mass spectrometry based chemistries and instrumentation to address these challenges. Aspects of this research include:
(i) systematic examination of the fundamental gas-phase ion chemistries of amino acids, peptides and proteins in order to determine the mechanisms and other factors influencing their fragmentation reactions;
The presence of post-translationally modified (PTM) amino acid residues within a peptide sequence often has a dramatic effect on the fragmentation behavior of its protonated ions under low energy collision induced dissociation (CID) tandem mass spectrometry (MS/MS) conditions. MORE
Mathivanan, S., Fahner, C.J., Reid, G.E., Simpson, R.J. ExoCarta (2012): database of exosomal proteins, RNA and lipids. Nucleic Acids Res. 2012 Jan;40(Database issue):D1241-4.
Kalcic, C.L., Reid, G.E., Lozovoy, V.V., Dantus, M. (2012) Mechanism elucidation for nonstochastic femtosecond laser-induced ionization/dissociation: from amino acids to peptides. J Phys Chem A. 116(11):2764-74.
Zhang, X., Fhaner, C.J., Ferguson-Miller, S.M., Reid, G.E. (2012) Evaluation of ion activation strategies and mechanisms for the gas-phase fragmentation of sulfoquinovosyldiacylglycerol lipids from Rhodobacter sphaeroides. Int J Mass Spectrom. 316-318:100-107.
Lu, Y., Zhou, X., Stemmer, P.M. and Reid, G.E. (2011) Sulfonium Ion Derivatization, Isobaric Stable Isotope Labeling and Data Dependent CID- and ETD-MS/MS for Enhanced Phosphopeptide Quantitation, Identification and Phosphorylation Site Characterization. J. Am. Soc. Mass Spectrom. 23(4):577-93. Invited article to ‘2011 ASMS Sanibel Conference’ Special Issue.
Opreanu, M., Tikhonenko, M., Bozack, S., Lydic, T.A., Reid, G.E., McSorley, K.M., Sochacki, A., Perez, G.I., Esselman, W.J., Kolesnick, R., Grant, M.B. and Busik, J.V. (2011) The unconventional role of acid sphingomyelinase in regulation of retinal microangiopathy in diabetic human and animal models. Diabetes. 60:2370-2378.
Palumbo, A.M., Smith, S.A., Kalcic, C.L., Dantus, M., Stemmer, P.M. and Reid, G.E. (2011) Tandem Mass Spectrometry Strategies for Phosphoproteome Analysis. Mass Spectrom. Rev. 30: 600-625.
Zhang, X., Hiser, C., Tamot, B., Benning, C., Reid, G.E. and Ferguson-Miller, S.M. (2011) Combined Genetic and Metabolic Manipulation of Lipids in Rhodobacter sphaeroides Reveals Non-phospholipid Substitutions in Fully Active Cytochrome c Oxidase. Biochemistry. 50: 3891-3902.
Zhang, X., Tamot, B., Hiser, C., Reid, G.E., Benning, C. and Ferguson-Miller, S.M. (2011) Cardiolipin-Deficiency in Rhodobacter sphaeroides Alters the Lipid Profile of Membranes and of Crystallized Cytochrome Oxidase, but Structure and Function are Maintained. Biochemistry. 50: 3879-3890.
Smith, S.A., Kalcic, C.L., Safran, K.A., Stemmer, P.M., Dantus, M. and Reid, G.E. (2010) Enhanced Characterization of Singly Protonated Phosphopeptide Ions by Femtosecond Laser-induced Ionization/Dissociation Tandem Mass Spectrometry (fs-LID-MS/MS). J. Am. Soc. Mass Spectrom. 21: 2031–2040.
J Griffitts, D Saunders, YA Tesiram, GE Reid, A Salih, S Liu, TA Lydic, JV Busik, JX Kang, RA Towner. (2010) Non-mammalian fat-1 gene prevents neoplasia when introduced to a mouse hepatocarcinogenesis model Omega-3 fatty acids prevent liver neoplasia. Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids. 1801:1133-1144.
Zhou, X., Lu, Y., Wang, W., Borhan, B. and Reid, G.E. (2010) ‘Fixed Charge’ Chemical Derivatization and Data Dependant Multistage Tandem Mass Spectrometry for Mapping Protein Surface Residue Accessibility. J. Am. Soc. Mass Spectrom. 21:1339-1351.
Wang, W-H., Palumbo, A.M., Tan, Y-J., Reid, G.E., Tepe, J.J. and Bruening, M.L. (2010) Identification of p65-associated Phosphoproteins by Mass Spectrometry after On-plate Phosphopeptide Enrichment Using Polymer-oxotitanium Films. J. Prot. Res. 9, 3005-3015.
Opreanu, M., Lydic, T.A., Reid, G.E., McSorley, K.M., Esselman, W.J. and Busik, J. (2010) Docosahexaenoic Acid Inhibits Cytokine Signaling in Human Retinal Endothelial Cells by Downregulating Sphingomyelinases. Invest. Ophthalmol. Vis. Sci. 51, 3253-3263.MORE